primescript tm fast rt reagent kit (TaKaRa)

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TaKaRa primescript tm fast rt reagent kit
Primescript Tm Fast Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 87884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescript+tm+rt+kit/PrimeScript+RT+Reagent+Kit/pmc13010969-83-33-43
Average 99 stars, based on 87884 article reviews

primescript tm fast rt reagent kit - by Bioz Stars, 2026-09

99/100 stars

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Isolation:

Article Title: Genome-wide systematic characterization of bZIP transcription factors and their expression profiles during stem in tumorous stem mustard
Article Snippet: .. Total RNA was isolated from whole seedlings of Brassica juncea var. tumida using the Plant MiniBEST RNA Extraction Kit (TaKaRa, Dalian, China). cDNA was synthesized with the PrimeScript TM RT Kit and gDNA Eraser (TaKaRa). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted on a Bio-Rad iQ5 system using SYBR®Prime qPCR Set (Fast HS) (BIOGROUND, Chongqing, China) with 14 gene-specific primer pairs ( ).The thermocycling conditions were as follows: 94 ∘ C for 20 s; 40 cycles at 94 ∘ C for 10 s, 60 ∘ C for 20 s, and 72 ∘ C for 30 s. The Bju Actin gene ( BjuB012485 ) was used as an internal reference ( ).

RNA Extraction:

Synthesized:

Article Title: Integrated Metabolome–Transcriptome Profiling Identifies JrMYB8 as a Repressor of Polyphenol Biosynthesis in Walnut ( Juglans regia L.)
Article Snippet: RNA quality and integrity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, https://www.thermofisher.com ) and gel electrophoresis. .. First-strand cDNA was synthesized using the PrimeScript TM RT Kit with a gDNA Eraser (TaKaRa) according to the manufacturer’s instructions. .. TB Green Premix Ex Taq TM (TaKaRa) was used to perform qRT-PCR on a real-time PCR instrument (QuantStudio 7 Flex Real-Time PCR system, Applied Biosystems, https://www.thermofisher.com ).

Article Title: Machine learning-based screening of potential diagnostic markers for prostate cancer with immune cell infiltration analysis and experimental validation
Article Snippet: RNA extraction and reverse transcription: total RNA was extracted using TRIzol TM reagent (15596026, Invitrogen, Carlsbad, CA, USA), and the purity (A260/A280 >1.8) was determined by NanoDrop 2000 (Thermo Fisher, Waltham, MA, USA). .. The cDNA was synthesized according to the instructions of PrimeScript TM RT Kit (RR047A, Takara, Kusatsu, Japan), and the reaction system contained 1 μg of RNA. .. APOBEC3C primer sequences were designed by NCBI Primer-BLAST (avoiding genomic DNA amplification across exon junction regions): Internal reference gene GAPDH: SYBR ® Green Master Mix (Vazyme, Q711-02) was used in QuantStudio 5 system (Applied Biosystems, Foster City, CA, USA) under the following reaction conditions: pre-denaturation at 95 °C for 5 min; 95 °C for 10 s, 60 °C for 30 s, for a total of 40 cycles.

cDNA Synthesis:

Article Title: High temperatures and microplastic enhanced inorganic phosphorus mineralization and phoD-harboring bacterial abundance in paddy soil.
Article Snippet: The DNA extraction was performed using the FastDNA Spin Kit for Soil (MP Biomedicals, LLC, Solon, OH, USA). .. Plant RNA was extracted using liquid nitrogen grinding and Trizol reagent, followed by cDNA synthesis with the PrimeScript TM RT kit (Takara, Japan). .. Quantitative PCR (qPCR) was performed on the LightCycler 96 system (Roche Applied Sciences, Germany) with SYBR Premix Ex Taq, using Rice Actin 1 as the internal reference for normalization (Livak and Schmittgen, 2001).

Reverse Transcription:

Article Title: The Down-Regulation of Lactoferrin Expression Induced by Cigarette Smoke can Induce Ferroptosis in COPD.
Article Snippet: Purpose Lactoferrin (LTF) plays a crucial role in iron homeostasis, immune response, and inflammation.. In the context of chronic obstructive pulmonary disease (COPD), LTF’s expression is significantly influenced by environmental factors, particularly cigarette smoke.. The pathological mechanism by which cigarette smoke regulates LTF and affects iron metabolism in COPD remains unclear.

Article Title: The CONSTANS-like 2 Gene Serves as a Pivotal Regulator of Flowering in Hemerocallis
Article Snippet: .. Total RNA was reverse-transcribed into double-strand cDNA using the PrimeScript TM RT kit with gDNA Eraser (Takara, Beijing, China), strictly adhering to the detailed instructions provided. ..

Article Title: ISX promotes tumor migration and invasion in lung cancer by upregulating COL1A1 in vitro
Article Snippet: Total RNA was extracted from these cells using the EZ-10 DNA-free RNA Mini-Preps Kit (Sangon Biotech Co., Ltd.) according to the manufacturer's instructions. .. A total of 1 μg RNA was then reverse transcribed into cDNA using a PrimeScript TM RT Kit with gDNA Eraser (Takara Bio, Inc.) according to the manufacturer's protocol. ..

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